B.R.S.M. Tet. Lett. Is other people

1. 

   schlarpi
   February 6th, 2013 - 13:32

   (HP)TLC is a quantitative method if done properly. Plates are spotted by a robot to accurately control droplet size and position. A reference standard (with a known assay) is used for calibration. Signals are detected by a plate reader. Overall it is the same procedure as for quantification by HPLC, but reference and samples can be run in parallel.

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     BRSM
     February 6th, 2013 - 14:17

     Okay, my bad for being an ignorant synthetic chemist. I’d never heard of that technique! Thanks!

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2. 

   Scooped
   February 7th, 2013 - 00:27

   You can do even fancier things with a TLC plate: check out this Org. Lett. (OL 2008, 10, 3493, with special note to Fig. 2 on page 2:

   http://pubs.acs.org/doi/abs/10.1021/ol8012759

   As synthetic organic chemist I say: If you can make your PI buying this thing…

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     Vinylogous
     February 10th, 2013 - 06:10

     I like how the abstract alleges that this technique will “significantly accelerate” the pace of organic chemistry research. Perhaps an oversell? But still cool!

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3. 

   FP
   February 16th, 2013 - 13:51

   Has anyone tried to scrape off a spot from the TLC plate, extract and take a mass? That would be great to do when you have confusing reactions or don’t have time to isolate all the compounds from a reaction.

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     octagonecologyst
     February 22nd, 2013 - 14:53

     yes i did. can come pretty handy. scratch the spot off, suspend the silica gel in isopropanol, filter through syringe filter, evaporate / redisolve if necessary, inject in LCMS, GCMS or whatever you like. cheers

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4. 

   utbiotech
   February 17th, 2013 - 13:21

   of course TLC can be quantitative, use tlc scanner.

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5. 

   Joe
   March 1st, 2013 - 00:30

   Nothing from Aldrich could surprise me now since I saw some products are Kosher grade.

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